pdxK [2019-06-27 14:43:32]

pyridoxine, pyridoxal, and pyridoxamine kinase

locus: BSU38020

pI: 4.92

mw: 28.87 kDa

protein length: 271 aa

gene length: 816 bp

function: biosynthesis of pyridoxal phosphate

product: pyridoxine, pyridoxal, and pyridoxamine kinase

essential: no

ec: 2.7.4.7

synonyms: ywdB,ipa-52r,thiD

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.12|Biosynthesis of pyridoxal phosphate]

Gene

Coordinates: 3,900,963 → 3,901,778

The protein

Catalyzed reaction/ biological activity

• ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine (according to Swiss-Prot)

Protein family

• thiD family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|97EDB4ACD64C5FA2E6014ED133DF18F602FF58BB|ThiD]

Structure

• [PDB|2I5B] [pubmed|16978644]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]

Expression and Regulation

Operons

• genes: [gene|FEDD589EA9982856730E809F21B7930F688FDC42|pdxK]
  description: [Pubmed|3100393]

Biological materials

Mutant

• BKE38020 (Δ[gene|FEDD589EA9982856730E809F21B7930F688FDC42|pdxK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTGAACCTCCATCA, downstream forward: _UP4_TAAAAAAAGGGGCTGCCTTA
• BKK38020 (Δ[gene|FEDD589EA9982856730E809F21B7930F688FDC42|pdxK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTGAACCTCCATCA, downstream forward: _UP4_TAAAAAAAGGGGCTGCCTTA

References

• 17012797,14973012,3100393,16978644